Recombinant proteins can be defined as recombinant DNA-encoded proteins cloned using an expression vector system to support gene expression and mRNA transcription. Recombinant DNA technology allows for the expression of mutant proteins through modifications. These proteins can be manipulated from native proteins. These proteins can be synthesized using genetic engineering, also known as gene-splicing or Recombinant DNA Technology. BTL Biotechno Labs has been an authorized supplier of Recombinant proteins and related products in India.
What are vectors?
Sometimes, purifying and expressing a protein can be difficult and time-consuming. Therefore, an additional tag is added along with the DNA sequence to aid in the purification and expression.
Recombinant Proteins Vs Natural Proteins - Advantages Of Recombinant Proteins
Research has shown that recombinant proteins have many advantages over natural proteins.
1. They can be synthesized using recombinant technologies
2. The protein yield is extremely high
3. Variations can be controlled from batch to batch.
4. Proteins do not contain animal contaminants
5. Modifying amino acid sequences is easy
6. It is possible to easily incorporate unnatural amino acids
Types of Expression Systems
Transfection of DNA vectors containing recombinant protein genes into a specific cell type is the strategy to express recombinant proteins. This culture then leads to the translation of the desired protein. The key to successful recombinant gene expression is choosing the right protein expression system. Many factors are considered when choosing the expression system, including target protein property, intended use, yield, cost, and risk. This is especially true for proteins with high post-translational modifications, high mol weight, and nuclear proteins. We are also an important distributor of ELISA kits in India.